Scientific Volume Imaging b.v.
Scientific Volume Imaging b.v.
Laapersveld 63
1213 VB Hilversum, The Netherlands
http://www.svi.nl
http://support.svi.nl/wiki

Scientific Volume Imaging

Pioneer in image Deconvolution, Visualization and Analysis

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Macrophage sample image

Macrophage fluorescently stained for tubulin (yellow/green), actin (red) and the nucleus (DAPI, blue) recorded with a Wide Field Microscope by Dr. James Evans, Whitehead Institute, MIT Boston MA, USA.

As deconvolved with Huygens Professional and visualized by FluVR's spectral fluorescence volume renderer:

macrophage

SVI_huygens_presentation_smaller

The 'Flying Macrophage':
Left part: original data
Right part: as deconvolved with Huygens Professional.

Data comparison

Original

A MIP of the raw data:

evans_macrophage_ORIGINAL_MIP

Restored

Deconvolved with the Huygens Software:

evans_macrophage_MLE_MIP

Close ups

Alternating nucleus or tubulin emphasized:

animationNucleusTubuline

closeup005 closeup008 closeup009

SFP renderings

Original: _4dtaALL_sfp

Deconvolved: 4daALLmle_sfp

Movie

Download hi-res AVI file: macrophage_cover_2parts_clean_0b.avi (3312 kB 2009-09-16 11:33)

High resolution top view

Preview:

macrophage_topView_preview

Download hi-res TIFF file: macrophage_topView_hiRes_lzw.tiff (1804 kB 2009-09-17 14:01)