Scientific Volume Imaging b.v.
This greatly depends on the optical paramaters, in particular on the NA and microscope type. Together with the wavelenghts and refractive indexes these determine the so called Nyquist sample distance, the maximum sampling distance at which all image information is captured. For a plot of the dependancy of the Nyquist rate on the NA and microscope type see the User guides, or go to Nyquist Rate. To calculate this figures online see the Nyquist Calculator.
While sampling at the Nyquist rate is a very good idea, it is in many practical situations hard to attain. In these cases larger sampling distances may be used. For confocal images sampling distance may be up to 1.7 of the Nyquist distance. When large pinholes are used, up to 2x.
Widefield data are more sensitive to undersampling; stay below 1.5. In case of low numerical apertures like 0.4 we recommend not to undersample in the axial direction.
The actual and the ideal sampling distances of an image can be seen in Huygens Essential right-clicking on the image thumbnail and choosing 'Show parameters'.
To compute the ideal Nyquist rate of an image in Huygens Professional, select the image and choose Edit -> Nyquist rate.
Keywords: sampling NA Nyquist numerical aperture
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac
Related products: Hu Ess Hu Pro Hu Script
