http://support.svi.nl/wiki 3D microscopy: imaging, restoration, visualization and analysis. http://support.svi.nl/wiki http://support.svi.nl/wiki/SVIc_50.png 57 36 3D microscopy: imaging, restoration, visualization and analysis. http://support.svi.nl/wiki/AdaptAndResampleThePsf 08 Mar 2010 11:55:06 +0100 http://support.svi.nl/wiki/AdaptAndResampleThePsf http://support.svi.nl/wiki/aaaa Describe aaaa here. (Remove this line). 23 Feb 2010 04:21:18 +0100 http://support.svi.nl/wiki/aaaa http://support.svi.nl/wiki/HuygensRemoteManagerHelpSnrEstimator Since version 1.2 of HRM, together with version 3.5.1 of HuygensCore, a SignalToNoiseRatio estimator is available that will return suggested Signal-to-Noise ratio (SNR or S/N) values, based on a representative image, to fill out the Restoration Task Settings form. The SNR estimator in HRM will return reasonable starting values for the type of images you want to deconvolve. You can further tune this SNR value by comparing the corresponding deconvolution results, to adapt the restoration to the experimental needs. The estimator works by letting you select a raw image, among the ones present at your directory in the HRM server, and automatically inspecting the level of noise in its different channels. A best-guess will be returned per channel, that you can write down and enter in the restoration task settings. (Values will not be entered automatically in the form). It is important to select an image for the estimation that is representative of the kind of images you want to deconvolve la [...] 18 Feb 2010 12:56:54 +0100 http://support.svi.nl/wiki/HuygensRemoteManagerHelpSnrEstimator http://support.svi.nl/wiki/HuygensRemoteManagerHelpHome The home page is the new entry point (as of version 1.2.0) of the Huygens Remote Manager that collects all actions in a nice layout. 17 Feb 2010 12:57:57 +0100 http://support.svi.nl/wiki/HuygensRemoteManagerHelpHome http://support.svi.nl/wiki/HuygensRemoteManagerHelpStatistics This page generates several graphical and tabular views of the stored statistics. While the administrator has full access to statistical data associated to any (completed) deconvolution jobs and can even download the whole statistics table from the HRM database to a text file for further processing (e.g. in some spreadsheet software), normal HRM users are only granted access to the statistical data associated to jobs launched by them. 17 Feb 2010 11:47:28 +0100 http://support.svi.nl/wiki/HuygensRemoteManagerHelpStatistics http://support.svi.nl/wiki/SamplingRates 15 Feb 2010 10:43:11 +0100 http://support.svi.nl/wiki/SamplingRates http://support.svi.nl/wiki/NewFeatures3.5 * Fixed a bug reading some TIFF series, specially in the BatchProcessor. * Fixed a bug in the BatchProcessor that made it fail in certain installations: tasks were not being processed. * The FileSeriesTool runs faster now, noticeably when loading many files over the network. * Fixed some conflicts in the Object Analyzer and Surface Renderer when changing the degree of transparency, for example when combining it with a MIP or a slicer. * Fixed a backwards compatibility problem with the -def option of the #setp# command in scripting. Bugs fixed in the Object Analyzer, deconvolution with many bricks, and others. Bug fixed in estimating the background. Some bugs fixed. This patched version corrects some bugs in the first release, like template exporting from Essential and STK file reading in Huygens Core. This is what you will find in the Huygens Suite release with Huygens Compute Engine version 3.5, to be released in the last quarter of 2009. * A brand new option called 'Object Stabilize [...] 12 Feb 2010 12:50:36 +0100 http://support.svi.nl/wiki/NewFeatures3.5 http://support.svi.nl/wiki/hhttp://www.bioquant.uni-heidelberg.de/about_us/how_to_find_us.html Describe here. (Remove this line). 29 Jan 2010 17:41:08 +0100 http://support.svi.nl/wiki/hhttp://www.bioquant.uni-heidelberg.de/about_us/how_to_find_us.html http://support.svi.nl/wiki/SamplingRate 22 Jan 2010 16:13:24 +0100 http://support.svi.nl/wiki/SamplingRate http://support.svi.nl/wiki/ObjectStabilizer This brand new tool can measure and correct for cell motion, thermal drift, shaking, and other types of movement (x-y-z translation and axial rotation). Both the measurement and subsequent stabilization are done in 3D and at sub-pixel level. The Stabilizer not only stabilizes 2D or 3D time series, but it also allows the alignment of slices within a 3D stack. The Object Stabilizer can be found in the Analysis menu in Huygens Essential and Professional. Images show the sum of four time frames of an apoptotic cell stained with fluorescent H2B nuclear protein. Time interval between slices is 300 seconds. Courtesy of M. Varecha and M. Kozubek, Masaryk University, Czech Republic. Stabilization over time works best if the time series has been deconvolved first. For time series there are three stabilization methods available: * Cross correlation. This can be considered an 'all-round method'. It can correct for both x-y-z translation and axial rotation. Adjacent time frames are compared and the [...] 29 Dec 2009 15:06:38 +0100 http://support.svi.nl/wiki/ObjectStabilizer